If you have read anything about Global25, you have met the phrase "get your coordinates from g25requests.app". This post explains what that portal is, what it costs as stated on its own site, what arrives when it is done, and what the row is good for, including the free tools and the full Global25 analysis at Ancestrify. It also explains the one alternative to going there yourself: ordering a G25 analysis with a raw file and letting the Coordinate Concierge obtain the same row on your behalf. And, because this is where most confusion lives, it explains why a row produced by a calculator or a "simulator" is not the same thing. If your question is really about ancestry modelling with a p-value rather than coordinates, that is the separate qpAdm analysis.
What the portal is#
Global25 is a principal-component-analysis space built by Davidski, the author of the Eurogenes blog, from a large set of ancient and modern genomes. Every sample in it is described by 25 numbers, its position on the first 25 principal components. A person's coordinates are their genome projected into that same space, and the projection can only be done by whoever holds the underlying dataset, which means Davidski. The portal at https://g25requests.app/ is his independent request service: the place where an individual submits a raw DNA file and receives a Global25 row computed against the real reference.
It is independent in the plain sense. It is not run by Ancestrify, not by any testing company, and not by any of the sites that host Global25 calculators. Ancestrify never computes Global25 coordinates and never will, because there is no way to do so without the reference dataset. What Global25 coordinates are, and how the space was built, is set out in What are Global25 coordinates.
Who runs it#
Davidski, the author of Eurogenes, who created the Global25 dataset and maintains it. The coordinates are produced by hand, per submission, against the current reference.
What you upload#
A raw-data export from a consumer testing company: 23andMe, AncestryDNA, MyHeritage, FamilyTreeDNA, LivingDNA and similar. The site states which formats it accepts; check it at the time of ordering, since accepted inputs can change. The upload is the same file you would give any raw-data service, and you should treat sending it as what it is, a transfer of your genotype data to a third party, with the same care you would apply to any other.
What comes back#
Two rows, each a name followed by 25 comma-separated numbers:
- a scaled row, in which each principal component has been multiplied by its eigenvalue so that the components carry weight proportional to the variance they explain;
- an unscaled row, the raw projection.
The scaled row is the one almost every Global25 tool expects, including all of ours. The unscaled row is worth keeping because some older calculators and some published population averages use it. The difference, and which to paste where, is covered in the coordinates post linked above.
A typical scaled row looks like this (illustrative, not a real person's coordinates):
Example_Kit,0.1263,0.1391,0.0587,-0.0424,0.0398,0.0134,-0.0021,0.0089,...
The file is small, a few hundred bytes, and it is yours. Keep it somewhere safe; you will paste it many times.
Fee and turnaround, as their site states#
Their site states a fee of 15 EUR per kit, and states a turnaround of 2 to 7 days. Both figures are Davidski's, published on his portal, and can change without notice; check the site before ordering rather than relying on this post. Ancestrify has no part in either figure.
What to do with the row#
Once you hold a scaled row, everything else is free until you want an analysis.
- The G25 distance calculator ranks the reference populations closest to your row by Euclidean distance across all 25 dimensions, in any of six eras, entirely in your browser.
- The G25 PCA viewer plots your row on curated era PCA views beside the ancient and modern populations.
- The G25 Authenticity Check flags a row that has been simulated, edited or has lost precision, which matters if the row came to you second-hand.
- The G25 admixture calculator fits your row to a chosen source panel and returns percentages with a fit distance.
None of those require an account for the in-browser computation. When you want the full Global25 analysis, 29.99 EUR, you paste the row at checkout and the analysis runs from it: distances, admixture and PCA across six eras, a video, and the report. Nothing in that pipeline recomputes or alters your coordinates; the row is the input, and it stays yours.
The alternative: let the Concierge obtain it#
If you would rather not manage the portal yourself, order the G25 analysis with your raw DNA file instead of a coordinate row and add the Coordinate Concierge for 15 EUR. With your explicit consent Ancestrify passes the file to Davidski's portal, receives the official scaled and unscaled rows, and runs the full analysis the moment they arrive. The 15 EUR is a pass-through of the portal's per-kit fee, and the rows are delivered to you to keep and reuse in any tool.
This is not a different source of coordinates. It is the same portal, the same row, ordered on your behalf so that one purchase covers both steps. The page that lays out both routes side by side is /get-g25-coordinates, and the step-by-step for doing it yourself is How to get Global25 coordinates.
Why simulated coordinates are not the same thing#
Several sites offer to produce "Global25-style" coordinates from a raw file for a lower fee, or for free, without the reference dataset. What they do is one of two things. Some fit your genome to a set of published population averages and back out a row that would sit at the fitted position; others run their own PCA on their own smaller panel and relabel the axes. In both cases the output has 25 numbers and pastes into the same tools, and in both cases it is not your position in the Global25 space, because no one but the dataset's holder can compute that.
The practical consequences are not subtle. A simulated row is typically pulled toward whatever population averages were used to fabricate it, so distance calculators report tighter matches to those populations than a real row would. Admixture calculators return cleaner percentages than the genome supports. And a PCA plot places the point where the fabrication put it. The Authenticity Check exists because such rows circulate widely and the people holding them often do not know.
The rule is simple: if the row did not come from g25requests.app, either directly or through the Concierge, it is not an official Global25 coordinate row, and any result computed from it inherits the fabrication. Fifteen euros and a few days is the cost of the real thing.
In short#
The portal is Davidski's, the fee and the timing are his and stated on his site, the row is yours, and Ancestrify's role is either nothing (paste your row at /g25) or an errand run with your consent (the Concierge at /get-g25-coordinates). If the question behind your search is "what ancient populations am I a mixture of, and can the answer be wrong", that is not a coordinates question at all; it is the qpAdm analysis, which merges the raw file itself and needs no Global25 row.
